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KMID : 0379120100380010029
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Korean Journal of Mycology
2010 Volume.38 No. 1 p.29 ~ p.33
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Effect of KGD1 Deletion on Cell Wall Biogenesis in Saccharomyces cerevisiae
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Kim Sung-Woo
Ahn Ki-Woong
Park Yun-Hee
Park Hee-Moon
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Abstract
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KGD1 gene was cloned by functional complementation of defects in β-1,3-glucan synthase activity of the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. We performed the gene disruption experiment to characterize the function of KGD1 gene, which encodes β-ketoglutarate dehydrogenase, in cell wall biosynthesis. The disruption of KGD1 showed the decreased growth rate, the increase of chitin synthases activity, alterations in cell wall composition, and increase of susceptibility to cell wall inhibitors such as Calcofluor white and Nikkomycin Z. These results suggested that KGD1 might be involved in cell wall biogenesis, especially the biosynthesis of β-1,6-glucan and chitin in S. cerevisaie.
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KEYWORD
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β-1, 3-glucan synthase, Cell wall composition, Chitin synthase, KGD1, Saccharomyces cerevisiae
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